TLE3 Monoclonal antibody, PBS Only

TLE3 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11A8

Cat no : 66083-1-PBS

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

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Validation Data Gallery

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  WB analysis of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66083-1-Ig (same clone as 66083-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 66083-1-Ig (same clone as 66083-1-PBS)

  IP result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66083-1-Ig (same clone as 66083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66083-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66083-1-PBS targets TLE3 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag17906
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 772 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC015729
• Gene Symbol: TLE3
• Gene ID (NCBI): 7090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )