TLE3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22094-1-AP

Synonyms

HsT18976, GRG3, GRG 3, ESG3, ESG

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Validation Data Gallery

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  WB analysis of HEK-293 using 22094-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22094-1-AP

  WB result of TLE3 antibody (22094-1-AP, 1:4000) with si-Control and si-TLE3 transfected HEK293 cells.

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  WB analysis of A431 using 22094-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22094-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22094-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 22094-1-AP

  IP result of anti-TLE3 (IP:22094-1-AP, 4ug; Detection:22094-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human lung cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22094-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (22094-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

22094-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag17300
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 772 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC015729
• Gene Symbol: TLE3
• Gene ID (NCBI): 7090
• RRID: AB_2878993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.

Publications

Species	Application	Title
mouse	WB,IF	Cells; Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.; Authors - Sangho Lee ; KD Validated