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TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22094-1-AP

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22094-1-AP is TLE3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17300
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 772 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
RRIDAB_2878993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 22094-1-APDownload protocol
IHC protocol for TLE3 antibody 22094-1-APDownload protocol
IF protocol for TLE3 antibody 22094-1-APDownload protocol
IP protocol for TLE3 antibody 22094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cells

Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.

Authors - Sangho Lee
  • KD Validated