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TLE1 Polyclonal antibody

TLE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22093-1-AP

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TLE1 Polyclonal antibody (22093-1-AP)

    WB analysis using 22093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22093-1-AP (TLE1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22093-1-AP targets TLE1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE1 fusion protein Ag17299
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 770 aa, 83 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC010100
Gene Symbol TLE1
Gene ID (NCBI) 7088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).

Protocols

Product Specific Protocols
WB protocol for TLE1 antibody 22093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols