TLE1 Polyclonal antibody

TLE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22093-1-AP

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

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Validation Data Gallery

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  WB analysis using 22093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22093-1-AP (TLE1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22093-1-AP targets TLE1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLE1 fusion protein Ag17299
• Full Name: transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 770 aa, 83 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC010100
• Gene Symbol: TLE1
• Gene ID (NCBI): 7088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE1 (Transducin-like enhancer protein 1) is a transcriptional corepressor and binds to a number of transcription factors. It can inhibit NF-kappa-B-regulated gene expression and the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (PMID: 10660609). TLE1 is highly expressed in postnatal brain (PMID: 29758293; 27852056).