TKTL2 Polyclonal antibody

TKTL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17135-1-AP

Synonyms

Transketolase-like protein 2, Transketolase like protein 2, transketolase like 2, EC:2.2.1.1

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Validation Data Gallery

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  WB analysis using 17135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17135-1-AP (TKTL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 17135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 17135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17135-1-AP (TKTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17135-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TKTL2 antibody (17135-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue
• Positive IHC detected in: rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17135-1-AP targets TKTL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TKTL2 fusion protein Ag10884
• Full Name: transketolase-like 2
• Calculated Molecular Weight: 626 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC028707
• Gene Symbol: TKTL2
• Gene ID (NCBI): 84076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transketolase-like 2 (TKTL2) plays an essential role in total transketolase activity and cell proliferation in cancer cells. TKTL2 is predicted to enable thiamine pyrophosphate binding activity and transketolase activity.