Transketolase Polyclonal antibody

• Featured Product
• KD/KO Validated

Transketolase Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 11039-1-AP

Synonyms

TKT, TK, EC:2.2.1.1

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Validation Data Gallery

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  WB analysis of Jurkat using 11039-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11039-1-AP (Transketolase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11039-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11039-1-AP (Transketolase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11039-1-AP (Transketolase antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11039-1-AP (Transketolase antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11039-1-AP (Transketolase antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of BxPC-3 using 11039-1-AP

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using Transketolase antibody (11039-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat liver tissue
• Positive IHC detected in: human lymphoma tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:100-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 21 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

11039-1-AP targets Transketolase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Transketolase fusion protein Ag1519
• Full Name: transketolase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC009970
• Gene Symbol: Transketolase
• Gene ID (NCBI): 7086
• RRID: AB_2245927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Publications

Species	Application	Title
human		Mol Psychiatry; A cross-species spatiotemporal proteomic analysis identifies UBE3A-dependent signaling pathways and targets.; Authors - Nikhil J Pandya
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	Proc Natl Acad Sci U S A; OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.; Authors - Ligong Chen
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB,IHC	Eur Respir J; Malignant pleural effusion cells show aberrant glucose metabolism gene expression.; Authors - Lin C-C CC ; KD Validated