Filter: AllWBIHCIF/ICC

WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67787-1-Ig (same clone as 67787-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.
IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

TK1 Monoclonal antibody, PBS Only

TK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67787-1-PBS

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Tk1b, Tk1a, TK 2, TK 1, EC:2.7.1.21

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67787-1-PBS targets TK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	TK1 fusion protein Ag8265 Predict reactive species
Full Name	thymidine kinase 1, soluble
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC007986
Gene Symbol	TK1
Gene ID (NCBI)	7083
RRID	AB_2918551
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04183
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).