TK1 Monoclonal antibody, PBS Only

TK1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1C2

Cat no : 67787-1-PBS

Synonyms

thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2

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Validation Data Gallery

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  WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67787-1-Ig (same clone as 67787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67787-1-Ig (TK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67787-1-Ig (same clone as 67787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67787-1-Ig (TK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 67787-1-Ig (same clone as 67787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TK1 antibody (67787-1-Ig, Clone: 1B1C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67787-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67787-1-PBS targets TK1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TK1 fusion protein Ag8265
• Full Name: thymidine kinase 1, soluble
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC007986
• Gene Symbol: TK1
• Gene ID (NCBI): 7083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).