TJAP1 Monoclonal antibody

TJAP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1F10

Cat no : 68495-1-Ig

Synonyms

DKFZp686F06131, PILT, Tight junction protein 4, TJAP1, TJP4

---

Validation Data Gallery

• 

  WB analysis using 68495-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HepG2 using 68495-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68495-1-Ig (TJAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, HSC-T6 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68495-1-Ig targets TJAP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TJAP1 fusion protein Ag11807
• Full Name: tight junction associated protein 1 (peripheral)
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 86-95 kDa
• GenBank Accession Number: BC064401
• Gene Symbol: TJAP1
• Gene ID (NCBI): 93643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).