TIMP2 Polyclonal antibody

TIMP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 26647-1-AP

Synonyms

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2

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Validation Data Gallery

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  IHC staining of mouse lung using 26647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse lung using 26647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 26647-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26647-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26647-1-AP (TIMP2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse lung tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26647-1-AP is TIMP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMP2 fusion protein Ag24436
• Full Name: TIMP metallopeptidase inhibitor 2
• Calculated Molecular Weight: 220 aa, 24 kDa
• GenBank Accession Number: BC052605
• Gene Symbol: TIMP2
• Gene ID (NCBI): 7077
• RRID: AB_2880587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.