TIMM8A Polyclonal antibody

• Featured Product
• KD/KO Validated

TIMM8A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11179-1-AP

Synonyms

TIM8A, TIM8, MTS, DFN1, DDP1

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Validation Data Gallery

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  WB analysis using 11179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11179-1-AP (TIMM8A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11179-1-AP

  IP result of anti-TIMM8A (IP:11179-1-AP, 4ug; Detection:11179-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 11179-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11179-1-AP (TIMM8A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 11179-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11179-1-AP (TIMM8A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11179-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11179-1-AP (TIMM8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

11179-1-AP targets TIMM8A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMM8A fusion protein Ag1661
• Full Name: translocase of inner mitochondrial membrane 8 homolog A (yeast)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC015093
• Gene Symbol: TIMM8A
• Gene ID (NCBI): 1678
• RRID: AB_2204687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
human	WB	Elife; Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.; Authors - Yilin Kang ; KO Validated
	WB	Cell Death Dis; Haploinsufficiency in the mitochondrial protein CHCHD4 reduces brain injury in a mouse model of neonatal hypoxia-ischemia.; Authors - Yanyan Sun
human	WB	Mol Genet Genomic Med; Functional analysis of a novel mutation in the TIMM8A gene that causes deafness-dystonia-optic neuronopathy syndrome.; Authors - Addison Neighbors
mouse	IF	Genes Dis; Cdc42 deletion yielded enamel defects by disrupting mitochondria and producing reactive oxygen species in dental epithelium; Authors - Jinxuan Zheng