TIMELESS Polyclonal antibody

• Featured Product
• KD/KO Validated

TIMELESS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14421-1-AP

Synonyms

TIMELESS1, timeless homolog (Drosophila), TIM1, TIM, Protein timeless homolog

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Validation Data Gallery

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  WB analysis using 14421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14421-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14421-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14421-1-AP (TIMELESS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14421-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14421-1-AP (TIMELESS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
• Positive IHC detected in: human spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below

Product Information

14421-1-AP targets TIMELESS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMELESS fusion protein Ag5767
• Full Name: timeless homolog (Drosophila)
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC050557
• Gene Symbol: TIMELESS
• Gene ID (NCBI): 8914
• RRID: AB_2201962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated
human	WB	Cell Death Dis; MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS.; Authors - Fangfang Li ; KD Validated
human	WB	Cell Prolif; Circadian clock genes promote glioma progression by affecting tumour immune infiltration and tumour cell proliferation.; Authors - Zeyu Wang
human	WB	bioRxiv; Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations; Authors - Sihang Zhou
human	WB,IHC	Gut Microbes; Exosomal-miR-129-2-3p derived from Fusobacterium nucleatum-infected intestinal epithelial cells promotes experimental colitis through regulating TIMELESS-mediated cellular senescence pathway; Authors - Shuchun Wei ; KD Validated
human	WB	Cell Signal; HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-β-catenin pathway; Authors - Zhaoxia Wang