TIM3 Recombinant antibody, PBS Only

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  WB analysis using 82836-1-RR (same clone as 82836-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82836-1-RR (TIM3 antibody) at dilution of 1:3500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 82836-1-RR (same clone as 82836-1-PBS)

  WB result of TIM3 antibody (82836-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIM3 transfected PC-3 cells. This data was developed using the same antibody clone with 82836-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82836-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82836-1-RR against Human TIM3 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

TIM3 {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA

Cat No. 82836-1-PBS

Clone No.5A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

T-cell immunoglobulin mucin receptor 3, T-cell immunoglobulin and mucin domain-containing protein 3, HAVcr-2, HAVCR2, CD366

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Product Information

82836-1-PBS targets TIM3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIM3 fusion protein Ag2459
• Full Name: hepatitis A virus cellular receptor 2
• Calculated Molecular Weight: 301 aa, 33 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC020843
• Gene Symbol: TIM3
• Gene ID (NCBI): 84868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDQ0
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).