Anti-Human TIGIT Rabbit Recombinant Antibody, PBS Only

TIGIT Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240423C7

Cat no : 98023-2-PBS

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

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Validation Data Gallery

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  FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98023-2-RR (same clone as 98023-2-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR, Clone: 240423C7) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98023-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98023-2-RR against Human TIGIT were performed. The affinity constant is 5.45 nM.

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Product Information

The immunogen of 98023-2-PBS is TIGIT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: T cell immunoreceptor with Ig and ITIM domains
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: NM_173799.4
• Gene Symbol: TIGIT
• Gene ID (NCBI): 201633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).