TIGAR Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01803-1

  Sandwich ELISA standard curve of MP01803-1, Human TIGAR Recombinant Matched Antibody Pair - PBS only. 85052-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17532. 85052-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01803-1

  The mean TIGAR concentration was determined to be 17.53 ng/mL in HCT 116 cell extract based on a 1.40 mg/mL extract load, 14.64 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load and 21.52 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP01803-1

  Cytometric bead array standard curve of MP01803-1, TIGAR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85052-1-PBS. Detection antibody: 85052-2-PBS. Standard: Ag17532. Range: 0.781-100 ng/mL.

TIGAR {ptg:Brand} Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85052-2-PBS

Clone No.242625D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

C12orf5, 242625D7, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR

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Product Information

85052-2-PBS targets TIGAR as part of a matched antibody pair:

MP01803-1: 85052-1-PBS capture and 85052-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TIGAR fusion protein Ag17532
• Full Name: chromosome 12 open reading frame 5
• Calculated Molecular Weight: 270 aa, 30 kDa
• GenBank Accession Number: BC012340
• Gene Symbol: TIGAR
• Gene ID (NCBI): 57103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ88
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.