TIGAR Polyclonal antibody

• Featured Product
• KD/KO Validated

TIGAR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22136-1-AP

Synonyms

C12orf5, TP53-induced glycolysis regulatory phosphatase, TP53-induced glycolysis and apoptosis regulator, Fructose-2,6-bisphosphatase TIGAR, EC:3.1.3.46

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Validation Data Gallery

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  WB analysis using 22136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22136-1-AP (TIGAR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse colon tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

22136-1-AP targets TIGAR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIGAR fusion protein Ag17532
• Full Name: chromosome 12 open reading frame 5
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012340
• Gene Symbol: TIGAR
• Gene ID (NCBI): 57103
• RRID: AB_2879003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TP53-induced glycolysis and apoptosis regulator (TIGAR), first reported in 2006, is a downstream target gene of p53 and an important factor involved in glycolysis and apoptosis . It is indispensable in metabolism and is involved in metabolic syndrome, including hyperglycemia, insulin resistance, alcoholic fatty liver and tissue ischemia. TIGAR is highly expressed in many cancer cells to promote cell survival.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa.; Authors - Yogita Kanan
mouse	WB	Neurobiol Dis; Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.; Authors - Amanda R Burmeister
human	WB	Cell Death Dis; MiR-652-5p elevated glycolysis level by targeting TIGAR in T-cell acute lymphoblastic leukemia.; Authors - Shan Liu
mouse	WB	Biochem Biophys Res Commun; Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway; Authors - Xiaoming Chai
human,mouse	WB	Phytother Res; Dual targeting of wild-type p53 and gut microbiota by Magnolol represses key metabolic process and kills CRC cells; Authors - Haixia Ji
human	WB	Cancer Sci; Impact of TP53-induced glycolysis and apoptosis regulator on malignant activity and resistance to ferroptosis in intrahepatic cholangiocarcinoma; Authors - Katsuya Toshida ; KO Validated