TIAM2 Polyclonal antibody

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26870-1-AP

Synonyms

T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2, TIAM 2, STEF, SIF and TIAM1-like exchange factor

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Validation Data Gallery

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  WB analysis using 26870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 26870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 26870-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, U2OS cells, mouse testis tissue, rat testis tissue, HEK-293 cells
• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26870-1-AP targets TIAM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIAM2 fusion protein Ag25469
• Full Name: T-cell lymphoma invasion and metastasis 2
• Observed Molecular Weight: 190-200 kDa
• GenBank Accession Number: BC137275
• Gene Symbol: TIAM2
• Gene ID (NCBI): 26230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.