TIAM1 Polyclonal antibody

TIAM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27694-1-AP

Synonyms

TIAM 1, TIAM1

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Validation Data Gallery

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  WB analysis using 27694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 27694-1-AP

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27694-1-AP (TIAM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HCT 116 cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27694-1-AP targets TIAM1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIAM1 fusion protein Ag26777
• Full Name: T-cell lymphoma invasion and metastasis 1
• Calculated Molecular Weight: 178 kDa
• Observed Molecular Weight: 178 kDa
• GenBank Accession Number: NM_003253
• Gene Symbol: TIAM1
• Gene ID (NCBI): 7074
• RRID: AB_2918127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma; Authors - Tianyu Han