TIAR Polyclonal antibody

• Featured Product
• KD/KO Validated

TIAR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17649-1-AP

Synonyms

TIAL1, TIA-1-related protein, TIA 1 related protein, TCBP, Nucleolysin TIAR

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Validation Data Gallery

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  WB analysis using 17649-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17649-1-AP (TIAR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17649-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17649-1-AP (TIAR antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

17649-1-AP targets TIAR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIAR fusion protein Ag11884
• Full Name: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: BC030025
• Gene Symbol: TIAR
• Gene ID (NCBI): 7073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites.. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)

Publications

Species	Application	Title
human	WB	Int J Mol Sci; HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.; Authors - Wenhui Zhao ; KD Validated