TIA1 Monoclonal antibody, PBS Only (Detector)

TIA1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C11F4

Cat no : 68486-1-PBS

Synonyms

RNA binding protein TIA 1, p40-TIA-1, p40 TIA 1, Nucleolysin TIA-1 isoform p40, Cytotoxic granule associated RNA binding protein TIA1

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Validation Data Gallery

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  WB analysis using 68486-1-Ig (same clone as 68486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68486-1-Ig (TIA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 68486-1-Ig (same clone as 68486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 68486-1-Ig (TIA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68486-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51061-1

  Cytometric bead array standard curve of MP51061-1, TIA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68486-2-PBS. Detection antibody: 68486-1-PBS. Standard:Ag2778. Range: 0.098-12.5 ng/mL.

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  Cytometric bead array standard curve of MP51061-2

  Cytometric bead array standard curve of MP51061-2, TIA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68486-3-PBS. Detection antibody: 68486-1-PBS. Standard:Ag2778. Range: 0.391-50 ng/mL.

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Product Information

68486-1-PBS targets TIA1 as part of a matched antibody pair:

MP51061-1: 68486-2-PBS capture and 68486-1-PBS detection (validated in Cytometric bead array)

MP51061-2: 68486-3-PBS capture and 68486-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIA1 fusion protein Ag2778
• Full Name: TIA1 cytotoxic granule-associated RNA binding protein
• Calculated Molecular Weight: 214 aa, 24 kDa, 43 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC015944
• Gene Symbol: TIA1
• Gene ID (NCBI): 7072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1 mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43.