THYN1 Monoclonal antibody

THYN1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12G3

Cat no : 68337-1-Ig

Synonyms

HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1

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Validation Data Gallery

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  WB analysis using 68337-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 68337-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of mouse testis using 68337-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68337-1-Ig (THYN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68337-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68337-1-Ig (THYN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells, U2OS cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, MOLT-4 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68337-1-Ig targets THYN1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: THYN1 fusion protein Ag8617
• Full Name: thymocyte nuclear protein 1
• Calculated Molecular Weight: 225aa,26 kDa; 166aa,19 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC006978
• Gene Symbol: THYN1
• Gene ID (NCBI): 29087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)