THYN1 Polyclonal antibody

• 

  WB analysis using 15867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15867-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15867-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15867-1-AP (THYN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15867-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15867-1-AP (THYN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15867-1-AP (THYN1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

THYN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15867-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

THY28KD, THY28, MY105, My0054, MDS012

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15867-1-AP targets THYN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THYN1 fusion protein Ag8481
• Full Name: thymocyte nuclear protein 1
• Calculated Molecular Weight: 225aa,26 kDa; 166aa,19 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC006978
• Gene Symbol: THYN1
• Gene ID (NCBI): 29087
• RRID: AB_2287179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P016
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)