THOP1 Recombinant antibody

THOP1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241607G8

Cat no : 84319-3-RR

Synonyms

THOP 1, MP78, MEPD_HUMAN, EP24.15, EC:3.4.24.15

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Validation Data Gallery

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  WB analysis using 84319-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84319-3-RR (THOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84319-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84319-3-RR against Human THOP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84319-3-RR is THOP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: THOP1 fusion protein Ag2268
• Full Name: thimet oligopeptidase 1
• Calculated Molecular Weight: 689 aa, 79 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC000135
• Gene Symbol: THOP1
• Gene ID (NCBI): 7064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides