THOC7 Polyclonal antibody

• Featured Product
• KD/KO Validated

THOC7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17881-1-AP

Synonyms

fSAP24, hTREX30, NIF3L1 binding protein 1, NIF3L1BP1, THO complex subunit 7 homolog, THOC7

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Validation Data Gallery

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  WB analysis using 17881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17881-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17881-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 17881-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 17881-1-AP (THOC7 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, HEK-293 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human kidney tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

17881-1-AP targets THOC7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THOC7 fusion protein Ag11795
• Full Name: THO complex 7 homolog (Drosophila)
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC065012
• Gene Symbol: THOC7
• Gene ID (NCBI): 80145
• RRID: AB_10548743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC7 is a member of the THO complex that associate with the mRNA export apparatus. It is required for efficient export of polyadenylated RNA. It acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Publications

Species	Application	Title
human	WB	Viruses; Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.; Authors - Toshie Sakuma ; KD Validated