THOC1 Polyclonal antibody, PBS Only

THOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10920-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10920-1-AP (same clone as 10920-1-PBS)

  IP result of anti-THOC1 (IP:10920-1-AP, 4ug; Detection:10920-1-AP 1:500) with HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10920-1-AP (same clone as 10920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10920-1-AP (THOC1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10920-1-AP (same clone as 10920-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (10920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10920-1-PBS in a different storage buffer formulation.

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Product Information

10920-1-PBS targets THOC1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THOC1 fusion protein Ag1352
• Full Name: THO complex 1
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC010381
• Gene Symbol: THOC1
• Gene ID (NCBI): 9984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.