THAP2 Polyclonal antibody

THAP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20853-1-AP

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 20853-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20853-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 20853-1-AP

  Immunofluorescent analysis of Hela cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 20853-1-AP

  Immunofluorescent analysis of HepG2 cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20853-1-AP targets THAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THAP2 fusion protein Ag14775
• Full Name: THAP domain containing, apoptosis associated protein 2
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC008358
• Gene Symbol: THAP2
• Gene ID (NCBI): 83591
• RRID: AB_10732806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THAP2, also named as THAP domain-containing protein 2, is a 228 amino acid protein, which contains one THAP-type zinc finger. Members of the THAP (thanatos-associated protein) family of proteins contain a well conserved DNA-binding domain known as the THAP-type zinc finger motif. Proteins containing the THAP-type zinc finger motif are commonly involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification

Publications

Species	Application	Title
rat	WB	Cancer Cell Int; The involvement of miR-100 in bladder urothelial carcinogenesis changing the expression levels of mRNA and proteins of genes related to cell proliferation, survival, apoptosis and chromosomal stability.; Authors - Denis R Morais
human	WB	Clinics (Sao Paulo); MicroRNA 100: a context dependent miRNA in prostate cancer.; Authors - Leite Katia R M KR