THAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

THAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12584-1-AP

Synonyms

THAP1

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Validation Data Gallery

• 

  WB analysis of HepG2 using 12584-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12584-1-AP

  WB result of THAP1 antibody (12584-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP1 transfected A549 cells.

• 

  WB analysis of mouse brain using 12584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12584-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12584-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12584-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12584-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12584-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 12584-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12584-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12584-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12584-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12584-1-AP (THAP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, A375 cells, SGC-7901 cells, mouse brain tissue, mouse heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

12584-1-AP targets THAP1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THAP1 fusion protein Ag3285
• Full Name: THAP domain containing, apoptosis associated protein 1
• Calculated Molecular Weight: 213 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC021721
• Gene Symbol: THAP1
• Gene ID (NCBI): 55145
• RRID: AB_2201672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.

Publications

Species	Application	Title
human	WB	Nat Genet; Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia.; Authors - Esther Meyer
mouse	WB,ChIP	Mol Cell; The dystonia gene THAP1 controls DNA double-strand break repair choice.; Authors - Kenta Shinoda ; KO Validated
human	WB	Brain; DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family.; Authors - Fubo Cheng ; KO Validated
mouse	WB	Ann Neurol; Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.; Authors - Gavarini Sophie S
human	WB	Ann Neurol; The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6).; Authors - Kaiser Frank J FJ
mouse	WB	Dev Cell; The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.; Authors - Dhananjay Yellajoshyula