TGN46 Monoclonal antibody, PBS Only

TGN46 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6D5

Cat no : 66477-1-PBS

Synonyms

Golgi marker, TGN38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2

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Validation Data Gallery

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  WB analysis of HeLa using 66477-1-Ig (same clone as 66477-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  WB analysis using 66477-1-Ig (same clone as 66477-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66477-1-Ig (TGN46 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66477-1-Ig (same clone as 66477-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66477-1-Ig (TGN46 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66477-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66477-1-PBS targets TGN46 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig, Mouse samples.

• Tested Reactivity: Human, Rat, Pig, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGN46 fusion protein Ag4470
• Full Name: trans-golgi network protein 2
• Calculated Molecular Weight: 447 aa, 47 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC028219
• Gene Symbol: TGOLN2
• Gene ID (NCBI): 10618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.