TGM4 Polyclonal antibody

TGM4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15847-1-AP

Synonyms

Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P

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Validation Data Gallery

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  WB analysis of mouse testis using 15847-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15847-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15847-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, HeLa cells, PC-3 cells, rat testis tissue
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15847-1-AP targets TGM4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGM4 fusion protein Ag8608
• Full Name: transglutaminase 4 (prostate)
• Calculated Molecular Weight: 684 aa, 77 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC007003
• Gene Symbol: TGM4
• Gene ID (NCBI): 7047
• RRID: AB_2287328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.