TGIF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TGIF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11522-1-AP

Synonyms

TGF-beta-induced transcription factor 2, TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2

---

Validation Data Gallery

• 

  WB analysis using 11522-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11522-1-AP (TGIF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11522-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11522-1-AP (TGIF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 11522-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using TGIF2 antibody (11522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, Daudi cells, HL-60 cells, Jurkat cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

11522-1-AP targets TGIF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGIF2 fusion protein Ag2085
• Full Name: TGFB-induced factor homeobox 2
• Calculated Molecular Weight: 237 aa, 26 kDa
• Observed Molecular Weight: 28 kDa and 30 kDa
• GenBank Accession Number: BC012816
• Gene Symbol: TGIF2
• Gene ID (NCBI): 60436
• RRID: AB_2202731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-beta-induced factor 2(TGIF2), is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. This antibody detected two forms of bands of about 28 and 30 kDa, and the upper band appears to result from increased phosphorylation (PMID: 11427533).

Publications

Species	Application	Title
mouse	WB	Cell Rep; Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells.; Authors - Bum-Kyu Lee
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska
human	WB	Exp Ther Med; Molecular mechanisms in vascular injury induced by hypertension: Expression and role of microRNA-34a.; Authors - Siguan Liu
mouse	WB	Brain Behav; Downregulation of TGIF2 is possibly correlated with neuronal apoptosis and autism-like symptoms in mice.; Authors - Jing Lei
human	WB	Front Immunol; TGIF2 is a potential biomarker for diagnosis and prognosis of glioma; Authors - Wan Zhang ; KD Validated