TGFBR2 Polyclonal antibody

TGFBR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 27212-1-AP

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

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Validation Data Gallery

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  WB analysis using 27212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27212-1-AP (TGFBR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, COLO320 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 27212-1-AP is TGFBR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGFBR2 fusion protein Ag25767
• Full Name: transforming growth factor, beta receptor II (70/80kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-80 kDa
• GenBank Accession Number: BC040499
• Gene Symbol: TGFBR2
• Gene ID (NCBI): 7048
• RRID: AB_2918119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; YWK-II/APLP2 inhibits TGF-β signaling by interfering with the TGFBR2-Hsp90 interaction; Authors - Amannisa Tuersuntuoheti
mouse	FC	Cell Commun Signal; The activation of PPARγ enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TβRII/IL-2Rα.; Authors - Yumeng Miao
human	WB	Reprod Sci; Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3.; Authors - Jing Zhang
mouse	WB	Front Pharmacol; Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway; Authors - Jiang Li
rat	WB	Int J Rheum Dis; JAK2 inhibitors improve RA combined with pulmonary fibrosis in rats by downregulating SMAD3 phosphorylation; Authors - Yimei Wei
mouse	WB	Acta Histochem; Inhibition of glutamine transporter ASCT2 mitigates bleomycin-induced pulmonary fibrosis in mice; Authors - Naqi Lian