TGF beta 2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

TGF beta 2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

48

Reviews (1)

Cat no : 19999-1-AP

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta 2-Specific, TGF beta2, TGFB2, TGF-beta 2, TGFβ2, TGF-β2

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Validation Data Gallery

• 

  WB analysis using 19999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19999-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19999-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19999-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 19999-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19999-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19999-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19999-1-AP (TGF-beta 2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, mouse lung tissue, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 41 publications below
• IHC: See 12 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Product Information

19999-1-AP targets TGF beta 2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transforming growth factor, beta 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_003238
• Gene Symbol: TGFB2
• Gene ID (NCBI): 7042
• RRID: AB_10646467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. This antibody is TGFB2 specific not detecting other TGFBs.

Publications

Species	Application	Title
human	WB	ACS Nano; Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling.; Authors - Na Huang
human	WB,IF	Cancer Res; Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.; Authors - Li-Yuan Yu-Lee ; KD Validated
human	WB	Cell Death Differ; USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.; Authors - Xiaohua Jie
human	WB	Dev Cell; Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.; Authors - Song-Chang Lin
human	IHC	Cancer Res; SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C.; Authors - Dan Liu
human	WB	Cancer Lett; ZEB1 represses biogenesis of circ-DOCK5 to facilitate metastasis in esophageal squamous cell carcinoma via a positive feedback loop with TGF-β.; Authors - Lingjiao Meng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kazu (Verified Customer) (12-14-2022)

A clear, single band at the expected molecular weight was observed by western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Feline ocular tissue

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