TGF Alpha Polyclonal antibody

TGF Alpha Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22308-1-AP

Synonyms

TGF-alpha, TGFA, TGF type 1, TFGA, ETGF

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Validation Data Gallery

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  IHC staining of human breast cancer using 22308-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:50.

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  IHC staining of human breast cancer using 22308-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:50.

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  IHC staining of human skin using 22308-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 22308-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 22308-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 22308-1-AP (TGF-alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

22308-1-AP targets TGF Alpha in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transforming growth factor, alpha
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: NM_003236
• Gene Symbol: TGF alpha
• Gene ID (NCBI): 7039
• RRID: AB_2879069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		J Cell Mol Med; CircRhoC promotes tumorigenicity and progression in ovarian cancer by functioning as a miR-302e sponge to positively regulate VEGFA.; Authors - Li-Li Wang
human	IHC	Mol Cancer; MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α.; Authors - Shuo Chen
human		Drug Des Devel Ther; Adenosine Inhibits Ovarian Cancer Growth Through Regulating RhoGDI2 Protein Expression.; Authors - Bing Xia
human	IHC	Int J Exp Pathol; Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma; Authors - Chao Zeng