TFPT Polyclonal antibody

TFPT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10097-2-AP

Synonyms

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

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Validation Data Gallery

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  WB analysis of K-562 using 10097-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10097-2-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10097-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10097-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, mouse liver tissue, PC-3 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10097-2-AP targets TFPT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFPT fusion protein Ag0131
• Full Name: TCF3 (E2A) fusion partner (in childhood Leukemia)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC001728
• Gene Symbol: TFPT
• Gene ID (NCBI): 29844
• RRID: AB_2240572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

Publications

Species	Application	Title
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	WB	EMBO J; Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.; Authors - Christian Schutt