TFPI Polyclonal antibody

TFPI Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31765-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 31765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31765-1-AP (TFPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31765-1-AP targets TFPI in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_006287.6
• Gene Symbol: TFPI
• Gene ID (NCBI): 7035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tissue factor pathway inhibitor (TFPI) is an anticoagulant protein produced primarily by the endothelium and megakaryocytes and alternative mRNA splicing generates two main forms, TFPIα and TFPIβ (PMID: 26149025, PMID: 16246254). TFPIα is a soluble form of TFPI. It is a ~46 kDa glycoprotein and consists of an acidic N-terminal regionfollowed by three tandem, Kunitz-type protease inhibitor domains and a basic C-terminal region. Based on protein mass, TFPIβ (28 kDa) is considerably smaller than TFPIα (36 kDa), but both migrate with the same apparent molecular mass (46 kDa) on sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDS-PAGE), suggesting a difference in post-translational modifications (PMID: 16246254). TFPI associated with LDLis 34 kDa and lacks a substantial portion of the carboxy terminus including at least a portion of the third Kunitz domain. TFPI associated with HDL is 41 kDa and is in part a mixed disulphide complex between TFPI and apolipoprotein All (PMID: 8979119). TFPI exerts its anticoagulant effects by inhibiting TF-FVIIa in a manner that is dependent on its inhibition of factor Xa (FXa) (PMID: 2927510).