TFG Monoclonal antibody, PBS Only

TFG Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B9

Cat no : 66916-1-PBS

Synonyms

FLJ36137, Protein TFG, TF6, TFG, TRK fused gene, TRK fused gene protein, TRKT3

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Validation Data Gallery

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  WB analysis using 66916-1-Ig (same clone as 66916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66916-1-Ig (same clone as 66916-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66916-1-Ig (same clone as 66916-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66916-1-Ig (same clone as 66916-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66916-1-Ig (same clone as 66916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66916-1-Ig (same clone as 66916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66916-1-Ig (same clone as 66916-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66916-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66916-1-PBS targets TFG in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TFG fusion protein Ag27697
• Full Name: TRK-fused gene
• Calculated Molecular Weight: 400 aa, 43 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC023599
• Gene Symbol: TFG
• Gene ID (NCBI): 10342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.