TFF2 Recombinant antibody

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  WB analysis of human urine using 83717-4-RR

  Human urine sample was subjected to SDS PAGE followed by western blot with 83717-4-RR (TFF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 83717-4-RR

  IP result of anti-TFF2 (IP:83717-4-RR, 4ug; Detection:83717-4-RR 1:1000) with mouse stomach tissue lysate 1360 ug.

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  FC experiment of PC-3 using 83717-4-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug TFF2 Recombinant antibody (83717-4-RR, Clone:240661G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83717-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83717-4-RR against Human TFF2 were performed. The affinity constant is below 1 pM.

TFF2 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), IP, ELISA

Cat No. 83717-4-RR

Clone No.240661G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Synonyms

SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

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Tested Applications

• Positive WB detected in: human urine sample
• Positive IP detected in: mouse stomach tissue
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83717-4-RR targets TFF2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFF2 fusion protein Ag4537
• Full Name: trefoil factor 2
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC032820
• Gene Symbol: TFF2
• Gene ID (NCBI): 7032
• RRID: AB_3671319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q03403
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).