TFF2 Polyclonal antibody

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

43

Reviews (1)

Cat no : 13681-1-AP

Synonyms

SML1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin, SP

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Validation Data Gallery

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  WB analysis of human stomach using 13681-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13681-1-AP

  human stomach sample were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13681-1-AP

  Human urine tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse stomach using 13681-1-AP

  IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug.

• 

  IHC staining of human stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13681-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse stomach using 13681-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human stomach tissue, human urine sample
• Positive IP detected in: mouse stomach tissue
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue, mouse stomach tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 25 publications below
• IF: See 19 publications below

Product Information

13681-1-AP targets TFF2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, hamster, gerbil
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFF2 fusion protein Ag4537
• Full Name: trefoil factor 2
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC032820
• Gene Symbol: TFF2
• Gene ID (NCBI): 7032
• RRID: AB_10598321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

Publications

Species	Application	Title
mouse	IF	Nature; A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.; Authors - Daniel T Montoro
mouse	IF	Nat Genet; Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development.; Authors - Lijiang Fei
mouse	IF,IHC	Nat Cell Biol; Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach.; Authors - Marc Leushacke
human,mouse	IHC,IF	Nat Cell Biol; A tumour-resident Lgr5+ stem-cell-like pool drives the establishment and progression of advanced gastric cancers.; Authors - A Fatehullah
human	IF	Gut; Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing.; Authors - Min Zhang
mouse	IF	Gastroenterology; Induction of gastric cancer by successive oncogenic activation in the corpus.; Authors - Daisuke Douchi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongxue (Verified Customer) (05-13-2022)

WB works.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

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