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TFEB Recombinant antibody, PBS Only

TFEB Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242093D11

Cat no : 84675-5-PBS

Synonyms

TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB, Alpha TFEB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFEB Recombinant antibody (84675-5-RR)

    WB analysis using 84675-5-RR (same clone as 84675-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84675-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84675-5-RR

    Affinity and Kinetic Characterization of 84675-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

Product Information

84675-5-PBS targets TFEB in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFEB fusion protein Ag4194
Full Name transcription factor EB
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC032448
Gene Symbol TFEB
Gene ID (NCBI) 7942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).