TFDP2 Polyclonal antibody

TFDP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11500-1-AP

Synonyms

Transcription factor Dp-2, DP2, Dp 2

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Validation Data Gallery

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  WB analysis of mouse heart using 11500-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:400) with SH-SY5Y cells lysate 1600ug.

• 

  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:600) with SH-SY5Y cells lysate 1360 ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, human brain tissue
• Positive IP detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11500-1-AP targets TFDP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFDP2 fusion protein Ag2054
• Full Name: transcription factor Dp-2 (E2F dimerization partner 2)
• Calculated Molecular Weight: 446 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC021113
• Gene Symbol: TFDP2
• Gene ID (NCBI): 7029
• RRID: AB_2202685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].

Publications

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E
human	IHC	Front Mol Biosci; Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data; Authors - Ya-Jun Deng