TFAM Recombinant antibody, PBS Only

TFAM Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 82745-1-PBS

Synonyms

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

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Validation Data Gallery

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  WB analysis using 82745-1-RR (same clone as 82745-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82745-1-RR (same clone as 82745-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82745-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

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Product Information

82745-1-PBS targets TFAM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFAM fusion protein Ag18299
• Full Name: transcription factor A, mitochondrial
• Calculated Molecular Weight: 246 aa, 29 kDa
• Observed Molecular Weight: 24-25 kDa
• GenBank Accession Number: BC126366
• Gene Symbol: TFAM
• Gene ID (NCBI): 7019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.