Transferrin/TF Recombinant antibody

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  WB analysis using 84386-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84386-5-RR (TF antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human urine using 84386-5-RR

  human urine sample were subjected to SDS PAGE followed by western blot with 84386-5-RR (TF antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Transferrin/TF Recombinant Antibody for WB, ELISA

Cat No. 84386-5-RR

Clone No.241769C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TF, transferrin, Serotransferrin, PRO2086, PRO1557

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Tested Applications

• Positive WB detected in: mouse spleen tissue, human urine sample, human placenta tissue, mouse liver tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84386-5-RR targets Transferrin/TF in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• RRID: AB_3671922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.