Transferrin Recombinant antibody

Transferrin Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5O7

Cat no : 81676-1-RR

Synonyms

TF, Serotransferrin, PRO2086, PRO1557, Beta-1 metal-binding globulin

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Validation Data Gallery

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  WB analysis using 81676-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81676-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 81676-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 81676-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma, mouse serum, rat serum, human urine
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:475-1:1900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81676-1-RR targets Transferrin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Transferrin fusion protein Ag11504
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.