TEX264 Polyclonal antibody

• Featured Product
• KD/KO Validated

TEX264 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 25858-1-AP

Synonyms

SIG11, testis expressed 264, TEX264, ZSIG11

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Validation Data Gallery

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  WB analysis using 25858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25858-1-AP

  WB result of TEX264 antibody (25858-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX264 transfected HEK-293 cells.

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  WB analysis of mouse testis using 25858-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25858-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U-251 using 25858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TEX264 antibody (25858-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, mouse brain tissue, HeLa cells, Neuro-2a cells
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 3 publications below

Product Information

25858-1-AP targets TEX264 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX264 fusion protein Ag23027
• Full Name: testis expressed 264
• Calculated Molecular Weight: 313 aa, 34 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC008742
• Gene Symbol: TEX264
• Gene ID (NCBI): 51368
• RRID: AB_2880272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Publications

Species	Application	Title
human	IF	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
mouse	WB,IF	ACS Chem Neurosci; Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation.; Authors - Zhiyu Wang ; KD Validated
mouse	WB	Biomedicines; Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice.; Authors - Mingzhu Yan
human	WB,IF	Mol Biol Cell; Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.; Authors - Andrew Kennedy
human	WB	Mol Biol Cell; CCPG1 recognizes ER luminal proteins for selective ER-phagy; Authors - Shunsuke Ishii
human	WB	Food Chem Toxicol; Excessive ER-phagy contributes to ochratoxin A-induced apoptosis; Authors - Huiqiong Deng