TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A11D6

Cat no : 67982-1-Ig

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

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Validation Data Gallery

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  WB analysis of rat testis using 67982-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 67982-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit testis using 67982-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67982-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67982-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67982-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat testis tissue, PC-3 cells, mouse testis tissue, rabbit testis tissue
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67982-1-Ig targets TEX14 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TEX14 fusion protein Ag13143
• Full Name: testis expressed 14
• Calculated Molecular Weight: 957aa,107 kDa; 1497aa,168 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC040526
• Gene Symbol: TEX14
• Gene ID (NCBI): 56155
• RRID: AB_2918731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.