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TET2 Polyclonal antibody

TET2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21207-1-AP

Synonyms

Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80



Tested Applications

Positive WB detected inA431 cells, HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21207-1-AP targets TET2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, goat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TET2 fusion protein Ag15583
Full Name tet oncogene family member 2
Calculated Molecular Weight 2002 aa, 224 kDa
Observed Molecular Weight 130 kDa, 250 kDa
GenBank Accession NumberBC150180
Gene Symbol TET2
Gene ID (NCBI) 54790
RRIDAB_10734584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

Protocols

Product Specific Protocols
WB protocol for TET2 antibody 21207-1-APDownload protocol
IHC protocol for TET2 antibody 21207-1-APDownload protocol
IF protocol for TET2 antibody 21207-1-APDownload protocol
IP protocol for TET2 antibody 21207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia

Authors - Zijuan Wu
humanWB

Sci Adv

The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins.

Authors - Zhijun Huang
mouseCoIP

Sci Adv

Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.

Authors - Maria A Hahn
mouseWB

Nat Commun

Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons.

Authors - Franziska R Traube
mouseWB

J Exp Med

TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity.

Authors - Xiang Sun
mouseWB

JACC Basic Transl Sci

Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation.

Authors - Wei Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Melanie (Verified Customer) (11-19-2019)

IHC of 5um thin skin sections was performed. A positive staining was observed in the skin epithelial layer as predicted by the human protein atlas. Citrate buffer pH6 was used for antigen retrieval

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Skin
TET2 Antibody Immunohistochemistry, validation (1:400 dilution) in Skin (Cat no:21207-1-AP)
FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western blot

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: K562