TET2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TET2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

67

Reviews (2)

Cat no : 21207-1-AP

Synonyms

Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80

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Validation Data Gallery

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  WB analysis using 21207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21207-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21207-1-AP

  IP result of anti-TET2 (IP:21207-1-AP, 3ug; Detection:21207-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21207-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TET2 antibody (21207-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 21207-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TET2 (21207-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: A431 cells, HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 55 publications below
• IHC: See 11 publications below
• IF: See 14 publications below
• CoIP: See 3 publications below
• ChIP: See 5 publications below

Product Information

21207-1-AP targets TET2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, goat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TET2 fusion protein Ag15583
• Full Name: tet oncogene family member 2
• Calculated Molecular Weight: 2002 aa, 224 kDa
• Observed Molecular Weight: 130 kDa, 250 kDa
• GenBank Accession Number: BC150180
• Gene Symbol: TET2
• Gene ID (NCBI): 54790
• RRID: AB_10734584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia; Authors - Zijuan Wu
human	WB	Sci Adv; The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins.; Authors - Zhijun Huang
mouse	CoIP	Sci Adv; Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.; Authors - Maria A Hahn
mouse	WB	Nat Commun; Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons.; Authors - Franziska R Traube
mouse	WB	J Exp Med; TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity.; Authors - Xiang Sun
mouse	WB	JACC Basic Transl Sci; Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation.; Authors - Wei Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melanie (Verified Customer) (11-19-2019)

IHC of 5um thin skin sections was performed. A positive staining was observed in the skin epithelial layer as predicted by the human protein atlas. Citrate buffer pH6 was used for antigen retrieval

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Skin

TET2 Antibody Immunohistochemistry, validation (1:400 dilution) in Skin (Cat no:21207-1-AP)

FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562

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