TES Polyclonal antibody

TES Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 10258-1-AP

Synonyms

Testin

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Validation Data Gallery

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  WB analysis of HEK-293 using 10258-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10258-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10258-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10258-1-AP (TES antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10258-1-AP

  IP result of anti-TES (IP:10258-1-AP, 4ug; Detection:10258-1-AP 1:1500) with K-562 cells lysate 4000ug.

• 

  IHC staining of human skin cancer using 10258-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10258-1-AP (TES antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10258-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10258-1-AP (TES antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, MCF-7 cells, mouse testis tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

10258-1-AP targets TES in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TES fusion protein Ag0379
• Full Name: testis derived transcript (3 LIM domains)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 38-48 kDa
• GenBank Accession Number: BC001451
• Gene Symbol: TES
• Gene ID (NCBI): 26136
• RRID: AB_2201712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Testin (TES) is a cytoskeleton-associated protein that localizes along actin stress fibers at cell-cell contact areas and at focal adhesion plaques. It interacts with a variety of cytoskeletal proteins and gets involved in regulation of cell adhesion and migration. TES is a putative tumor suppressor and reduced expression of TES has been reported in various cancers. In addition, TES is a marker for Sertoli-Germ Cell junction and is useful to monitor the disruption of intercellular junctions in the testis.

Publications

Species	Application	Title
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang
human,mouse	IHC	Chem Biol Interact; The JNK/P38 signaling pathway activated by Testin protects the intestinal epithelial barrier against Crohn's disease-like colitis; Authors - Xue Song
human	WB	Front Mol Biosci; Development and Verification of a Hypoxic Gene Signature for Predicting Prognosis, Immune Microenvironment, and Chemosensitivity for Osteosarcoma.; Authors - Fengfeng Wu
mouse	WB	Biol Reprod; The Aryl Hydrocarbon Receptor Is Important for Proper Seminiferous Tubule Architecture and Sperm Development in Mice.; Authors - Hansen Deborah A DA
human	WB	Oncol Lett; Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma.; Authors - Giedrius Steponaitis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-07-2019)

The antibody works well. The first lane are cells expressing GFP-TES and the second lane is just wildtype cells. The antibody recognizes both endogenous and exogenous TES.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

TES Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10258-1-AP)