TERT Polyclonal antibody

TERT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27586-1-AP

Synonyms

EST2, hEST2, TCS1, Telomerase catalytic subunit, TERT, TP2, TRT

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Validation Data Gallery

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  WB analysis using 27586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27586-1-AP (TERT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

27586-1-AP targets TERT in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TERT fusion protein Ag26270
• Full Name: telomerase reverse transcriptase
• Calculated Molecular Weight: 127 kDa
• Observed Molecular Weight: ~100 kDa
• GenBank Accession Number: NM_001193376
• Gene Symbol: TERT
• Gene ID (NCBI): 7015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Telomerase reverse transcriptase (TERT) is a catalytic subunit of telomerase. TERT plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. TERT is one of two major components of the larger telomerase complex, which extends telomeres by adding specific short repetitive DNA sequences. Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. The calculated MW of TERT is 127 kDa, 27586-1-AP can detect a band around 100 kDa.

Publications

Species	Application	Title
human	WB	J Tissue Eng; Establishing an hTERT-driven immortalized umbilical cord-derived mesenchymal stem cell line and its therapeutic application in mice with liver failure; Authors - Qi Chen
mouse	WB	J Inflamm Res; FTO Stabilizes MIS12 to Inhibit Vascular Smooth Muscle Cell Senescence in Atherosclerotic Plaque; Authors - Jingzhao Sun
human	WB	Transl Cancer Res; Effect and mechanism of curcumin on colon cancer cell senescence through early growth response 1 (EGR1); Authors - Dan Xu