TERF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TERF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22020-1-AP

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

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Validation Data Gallery

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  WB analysis of Jurkat using 22020-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 22020-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22020-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22020-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22020-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 22020-1-AP

  IP result of anti-TERF2 (IP:22020-1-AP, 4ug; Detection:22020-1-AP 1:500) with K-562 cells lysate 4000ug.

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  IHC staining of human gliomas using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human gliomas tissue, rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

22020-1-AP targets TERF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TERF2 fusion protein Ag16798
• Full Name: telomeric repeat binding factor 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 65-69 kDa
• GenBank Accession Number: BC024890
• Gene Symbol: TERF2
• Gene ID (NCBI): 7014
• RRID: AB_10973675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRF2, also named as TRBF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TRF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TRF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Inhibition of TRF2 Leads to Ferroptosis, Autophagic Death, and Apoptosis by Causing Telomere Dysfunction; Authors - Qiuhui Yang ; KD Validated
human	WB	Cell Mol Biol Lett; MCCC2 is a novel mediator between mitochondria and telomere and functions as an oncogene in colorectal cancer; Authors - Wanjun Liu