TEKT2 Polyclonal antibody

TEKT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13518-1-AP

Synonyms

h tektin t, TEKT2, TEKTB1, Tektin 2, tektin 2 (testicular), Tektin B1, TEKTIN T, Testicular tektin

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Validation Data Gallery

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  WB analysis using 13518-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13518-1-AP (TEKT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13518-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13518-1-AP (TEKT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human trachea using 13518-1-AP

  Immunohistochemical analysis of paraffin-embedded human trachea using 13518-1-AP (TEKT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human trachea using 13518-1-AP

  Immunohistochemical analysis of paraffin-embedded human trachea using 13518-1-AP (TEKT2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse brain tissue
• Positive IHC detected in: human trachea tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13518-1-AP targets TEKT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEKT2 fusion protein Ag4457
• Full Name: tektin 2 (testicular)
• Calculated Molecular Weight: 430 aa, 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC035620
• Gene Symbol: TEKT2
• Gene ID (NCBI): 27285
• RRID: AB_2203328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB,IP	Cell Mol Life Sci; Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella; Authors - Xin-Yan Geng
mouse	WB	Front Cell Dev Biol; TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.; Authors - Jingjing Zhang
bovine	WB,IHC	Asian-Australas J Anim Sci; Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility.; Authors - Zhaocheng Xiong
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma
mouse	IF	Dev Dyn; Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects; Authors - Pei-Yi Pan