TEKT1 Polyclonal antibody

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  WB analysis of human brain using 18968-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18968-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 18968-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18968-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18968-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18968-1-AP

  Immunofluorescent analysis of HepG2 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 18968-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

• Featured Product
• KD/KO Validated

TEKT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18968-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Tektin-1

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Tested Applications

• Positive WB detected in: human brain tissue, human testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

18968-1-AP targets TEKT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEKT1 fusion protein Ag5173
• Full Name: tektin 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC014599
• Gene Symbol: TEKT1
• Gene ID (NCBI): 83659
• RRID: AB_2203324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969V4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB,IP	Cell Mol Life Sci; Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella; Authors - Xin-Yan Geng
human	WB,IF	Hum Mol Genet; Functional characterization of tektin-1 in motile cilia and evidence for TEKT1 as a new candidate gene for motile ciliopathies.; Authors - Rebecca Ryan ; KD Validated
mouse	IF	Dev Dyn; Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects; Authors - Pei-Yi Pan