TEAD4 Recombinant antibody

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  WB analysis of MDA-MB-231 using 84932-4-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 84932-4-RR

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84932-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TEAD4 antibody (84932-4-RR, Clone: 242496B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84932-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84932-4-RR against Human TEAD4 were performed. The affinity constant is 0.205 nM.

TEAD4 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84932-4-RR

Clone No.242496B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TEA domain family member 4, TCF13L1, RTEF1, hRTEF 1B, EFTR 2

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, BGC-823 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84932-4-RR targets TEAD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated Molecular Weight: 434 aa, 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC015497
• Gene Symbol: TEAD4
• Gene ID (NCBI): 7004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15561
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.